Mimi Wachendorf, BS

I isolated and digested adult cardiac cells using a Langendorff perfusion apparatus. After collecting the separated ventricular and atrial cells, I plated them aiming for long-term viability. My goal was to orient them into a 3D anisotropic culture to mimic the structure of the adult human heart. For measuring cardiac integrity, the cultures were subjected to immunostaining, Western Blots, and optical mapping.

mtw4@duke.edu